KMID : 0359319870270010041
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Korean Journal of Veterinary Research 1987 Volume.27 No. 1 p.41 ~ p.45
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Phytomitogen Induced Blast Transformation of Guinea Pig
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Abstract
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The present study was carried out to investigate the optimal conditions on blastogenesis of guinea pig lymphocytes. A microculture system in conjuction with a semiautomatic multiple sample harvester was used to study the in vitro optimal condition of guinea pig lymphocytes.
Careful analysis of lymphocyte transformation to confanavalin A (Con A) and lipopolysaccharide (LPS) mitogen determined optimal conditions as :
(a) 10% fetal bovine serum in RPMI-1640 medium
(b) 48-hour culture period.
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